Plant Tissue Cult. 7(1) : 1-11, 1997 (June)

High Frequency Regeneration of Cymbopogon polyneuros Stapf as Controlled Physiochemical Factors and their Chromosomal Stability

A. B. Das*, R. Mallick and A. Chatterjee

Centre of Advabnced Study, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road 700 019, India

Key words: Cymbopogom ployneuros, Organogenesis, Plant regeneration

Abastract

High frequency shoots were noticed from leaf base callus of Cymbopogon polyneuros in MS medium supplemented with 4.5 mg/1 6-benzyl-aminopurine (BA), L-arginine, adenine and a low level on NAA. About 80 ?85 shoots buds were obtained from ca 200 mg of callus per culture. The individual shoots produced root in the presence of 0.5 ?3 mg/1 indole 3-butyric acid (IBA) or indole 3-butyric acid containing potassium salt (KIBA). Regenerated plants were cytologically and phenotypically stable. Regenerants were transplanted into soil and subsequently transferred to field for studying various morphological and biochemical characteristics. The results of one year of field trials showed that organogenetically derived plants were more uniform in all the characteristics as compared with the plants raised through slips by conventional propagation practices.

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