Plant Tissue Cult. & Biotech. 33(1): 25-36, 2023 (June)

General

Optimizing Micropropagation Protocol of Rose (Rosa hybrida L.) cv. ‘Double Delight’

Kazi Tanbir Rahman, Snigdha Sarker, Md. Raihan Iqbal Raju^* and Mohd. Talim Hossain

Department of Botany, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

Key words: Micropropagation, Rose cultivar, Rosa hybrida, Direct and indirect organogenesis

Abastract

An experiment was conducted to develop a suitable in vitro plant regeneration technique for Rosa hybrida L. cv. ‘Double Delight’ using nodal segments and leaf tissues as explants through direct and indirect organogenesis. Aseptic explants were cultured onto gelled MS medium contained various strengths of plant growth regulators alone and in combinations for callus and direct shoot induction. MS medium containing 2.0 mg/l BAP was found to be the most effective for direct shoot induction from nodal explants (87.5%) as it produced the maximum number of shoots per explant (4.20 ± 0.37). The highest callus induction frequency was found to be 80% in leaf tissue, 72% in nodal segments, and 96% in nodal segments having young leaf of in vitro raised plantlets in MS medium containing 2.5 mg/l 2, 4-D. The best percentage of indirect shoot organogenesis (93.33%) with maximum number and length of shoot were found when the different explantderived callus was transferred in MS medium supplimented with 3.0 mg/l BAP. The direct and indirectly induced shoots were multiplied on MS medium containing 3.0 mg/l BAP, where the average number and length of shoots per culture were 7.20 ± 0.80 and 5.22 ± 0.47 cm, respectively. Maximum rooting (80%) was observed in ½-strength of gelled MS medium containing 15.0 mg/l sucrose with 1.0 mg/l IBA. Plantlets with the proper root system were then placed in a polybag with a 1:1:1 ratio of sand, garden soil, and compost, and they had a survival rate of about 76%.

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