Plant Tissue Cult. & Biotech. 33(1): 1-8, 2023 (June)

General

In vitro Regeneration of an Endangered Medicinal Orchid Dendrobium crepidatum Lindl. & Paxton through the Development of Protocorm like Bodies

Ashok N. Pyati

Department of PG Botany, Maharani's Science College for Women, JLB Road, Mysore, Karnataka, India

Key words: Regeneration, Dendrobium, Protocorm like bodies, Callus, Plant growth regulators

Abastract

A protocol for in vitro regeneration of an endangered medicinal orchid Dendrobium crepidatum was established mainly through the formation of indirect protocorm like bodies (PLBs). The PLBs procured from in vitro growth cultures was assessed in ½ MS medium alone and in combinations with 6-Benzyl amino purine (BAP), Kinetin (Kn) and α-Naphthalene acetic acid (NAA). The callus tissue was induced from longitudinally bisected segments of PLBs. The optimum percentage of callus formation was obtained on ½ MS medium supplemented with BAP (1.0 mg/l) and a combination of BAP (1.0 mg/l) with NAA (0.5 mg/l), where 64.3% and 83.3% of callus was formed respectively. Formation of PLBs were achieved when callus mass was transferred onto the combinations of BAP + NAA and Kn + NAA in ½ MS medium. The optimum percentage (91.6%) of PLBs were regenerated in the combination of BAP with NAA. These PLBs differentiated into intact plantlets. Plantlets of about 3 cm in length with 4-5 roots were transplanted into pojng mixture where the highest rate of survival was 93.3% in a green house.

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