Plant Tissue Cult. & Biotech. 32(2): 127-136, 2022 (December)

General

Indirect Organogenesis from in vitro Derived Leaf and Internodes of Coccinia cordifolia (L.) Cogn. - An Important Medicinal Climber

Md. Taufiqur Rahman Sarkar^*1 and Mohammad Firoz Alam

Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Key words: Callus induction, Indirect organogenesis, Regeneration, Acclimatization.

Abastract

An efficient in vitro regeneration protocol was developed via callus from the explants of leaf and internode of Coccinia cordifolia (L.) Cogn., an important medicinal climber. Among the different combinations of auxins and cytokines used, BAP with NAA were found highly effective for induction of callus from in vitro derived leaf disc and internode explants. Maximum callus formation rates were recorded on MS with 1.0 mg/l BAP and 0.1 mg/l NAA for both leaf disc and internode explants. The best organogenesis via callus was obtained on MS with 2.0 mg/l BAP and 0.5 mg/l NAA. The highest numbers of shoots regenerated per culture were 6.5

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