Plant Tissue Cult. 6(2) : 95-98, 1996

In vitro Propagation and Ex vitro Acclimatization of ?Sahara F₁ Cucumber (Cucumis sativus L.)

Rida A. Shibli and Mohammed A. Ajlouni

Faculty of Agriculture, Jordan University of Science and Technology, P.O. Box 3030, Irbid-Jordan

Key words: Cucumis sativus, Tissue culture, Growth regulators

Abastract

Single node stem cuttings of ?Sahara F₁? cucumber (Cucumis sativus L.) were in vitro grown on MS medium supplemented with (0.0, 0.5, 1.0, 1.5, 2.0 mg/1) of the either benzyladenine (BA), kinetin (Kn), gibberellin (GA₃), indoleacetic acid (IAA) or indolebutyricacid (IBA). IAA and IBA showed significant enhancement of rooting, while Kn and GA₃ promoted shoot proliferation. BA and Kn induced callus formation compared with the control. Ninety per cent of the rooted microshoots survived the in vivo acclimatization conditions, grew, flowered and set fruits number under the glasshouse conditions.

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