Plant Tissue Cult. & Biotech. 31(1): 1-12, 2021 (June)

Impact of Encapsulation on Plantlet Regenerationfrom in vitro Grown Shoot tips of Citrus aurantifolia (Lime)

Priyanka Sharma and Bidhan Roy

Department of Seed Science and Technology, Faculty of Agriculture, Uttar Banga Krishi Viswavidyalaya, Pundibari, Cooch Behar, 736165, West Bengal, India

Key words: Citrus aurantifolia, Germination, In vitro mass propagation, Plant propagules, Plant growth regulators, Synthetic seed

Abastract

In order to conserve diverse species of citrus,an experiment on in vitro micropropagation and production of synthetic seeds from in vitro regenerated plant propagules of the species; Citrus aurantifolia (Lime) was carried out in which shoot tips were found to be suitable for excapsulation of artificial seeds. Highest rate of germination was obtained from the shoot tips when MS was supplemented with 1mg/lBAP. Beaded shoot tips produced maximum germination (81.43%).Germinated synthetic seeds with well established roots and shoots were were taken out from the culture bottles and transferred in plastic cups containing a mixture of sterile soil:sandand farmyard manure at a ratio of 1:1:1.Seedlings were further shifted in earthen pots and kept in a partial shed net house for 7 days. Those seedlings were finally transferred under the field conditions for acclimatization.

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