Plant Tissue Cult. & Biotech. 31(1): 1-12, 2021 (June)

Micropropagation of Citrus indica Tanakausing Shoot tips from Mature Trees

Kiran Chhetri, Binu Mathew and Lolly S. Pereira

Department of RDAP, North-Eastern Hill University, Tura Campus, Tura, West Garo Hills, Meghalaya, 794002, India.

Key words: Citrus indica, shoot tips, in vitro propagation.

Abastract

A study was conducted to standardize a protocol for in-vitro direct regeneration and mass multiplication of Citrus indica Tanaka using shoot tip explants excised from mature trees. Shoot tips were inoculated in MS medium supplemented with varying concen-trations and combinations of cytokinins and auxins. MS media when fortified withBAP 0.5 mg/l and 0.5 mg/l BAP + 1.0 mg/lKn were found to be the best treatments for shoot initiation while MS supplemented with 1 mg/l IBA; 0.5 mg/l NAA + 0.5 mg/l IBA and 0.5 mg/l NAA + 0.5 mg/l IAA were the best treatments for root induction. Among the different media used for hardening, 100 % survivability was obtained when plantlets were hardened using vermicompost as the potting medium. Subsequently, these plantlets were transferred to larger pots and acclimatization was achieved gradually in outdoor conditions.

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