Plant Tissue Cult. & Biotech. 31(1): 1-12, 2021 (June)

Effects of Bavistinand Cefotaximeon in vitro Contaminant free Shoot Regeneration of Ruelliatuberosa L.

P.V. ChaithanyaLakshmi, C.M. NarendraReddyand B. Srinivas

Department of Biotechnology,School of Herbal Studies and Naturo Sciences,Dravidian University, Kuppam, Andhra Pradesh-517426, India

Key words: Ruelliatuberosa, Bavistin, Cefotaxime, Decontamination

Abastract

In general, antimicrobial agents areoften used in micropropagation techniques to obtain contaminant free clones. The objective of the present study was to evaluate the effects of bavistin and cefotaximeonproducing contaminant free plants of Ruelliatuberosaculturedon MS supplemented with phytohormones. Field grown nodal explants of Ruelliatuberosa was used to regenerate entire plants viadirect organogenesis. Among thedecontaminantstested, the fungicide bavistinalong withhigher concentration of BAP (2.0 mg/l) and lower concentration of NAA (1.0 mg/l)wasthe most effective in regeneration and producing contaminant free shoots from cultured explants. This fungicide at 300mg/lminimised fungal contamination with survival rate of 54%. While the addition of decontaminantcefotaxime at low concentration (200 mg/l) along with same concentration of BAP and NAA stimulated the bud formation and controlled the bacterial contamination.However,its increasing concentration adversely affected the survival rate of Ruelliatuberosa. These findings clearly showed that low concentrations of bavistin and cefotaxime were not only non-toxic but also facilitated bud regeneration. The results achieved showed the decisive role not only of the use of successful fungicides and antibiotics, but also of their sufficient doses were very important in reducing contamination andhelping multiple shoot proliferation.

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