Plant Tissue Cult. & Biotech. 30(2): 189-197, 2020 (December)

Asymbiotic Seed Germination of Thunia alba, a Variable Orchid in an Isolated Karst Hill of Xishuangbanna, Yunnan, China

Zi-Yu Zhou, Ju Li, Xi-Hui Peng and Sven Landrein^*

Xishuangbanna Tropical Botanical Garden XTBG, Horticulture Department, Yunnan, Xishuangbanna, Mengla, Menglun Town, 666303 China

Key words: Ex situ conservation, Thunia alba, A variable orchid

Abastract

Asymbiotic seed germination of Thunia alba (Lindley) H.G. Reichenbach was tested on different media in order to propagate and preserve its genetic diversity. In addition different plant hormones were used in the similar germination experiments. Results from these experiments revealed that Knudson medium (KC) had the best germination ability but Mitra and modified Murashige and Skoog (MS) also showed good responses for growth and root formation in the seedling after eight weeks. Coconut water (CW) was the most efficient for promoting germination and growth, addition of 0.046

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