Plant Tissue Cult. & Biotech. 30(1): 33-45, 2020 (June)

In vitro Plant Regeneration of Rauvolfia tetraphylla L.: A Threatened Medicinal Plant

Rabbi Hoque, Rita Sarah Borna, M. Imdadul Hoque and R. H. Sarker

Plant Breeding and Biotechnology Laboratory, Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Key words: Rauvolfia tetraphylla, In vitro seed germination, Regeneration, Seedling development

Abastract

Poor seed germination is the main obstacle for seed propagation of Rauwolfia tetraphylla L. in nature. The high viability (83.1%) of seeds in nature and the presence of viable embryo indicate that viability is not the only reason behind poor seed germination of this valuable medicinal plant. However, to overcome the problem of poor seed propagation, an efficient protocol has been developed for R. tetraphylla. Among the various treatments the maximum rate of ex vitro seed germination (13.33%) was found when the seeds were treated with 100 ppm of GA3. Response of in vitro germination was found to vary under different conditions. Not only that the rate was much higher compared to ex vitro germination. The rate of seed germination was found to be 78% in MS without PGR (plant growth regulator) supplements, while it was 70 and 78% in cotton bed under light and dark, respectively. It was 80% in incubator at 37

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