Plant Tissue Cult. & Biotech. 29(2): 245-255, 2019 (December)

In Planta Genetic Transformation of Mungbean (Vigna radiata (L.) Wilczek) with Marker Gene

Mohammad Mahmood Hasan, Sujay Kumar Bhajan, M. Imdadul Hoque, R. H. Sarker and Mohammad Nurul Islam^*

Plant Breeding and Biotechnology Laboratory, Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Key words: Mungbean, In planta transformation, Gus gene, pBI121, Recalcitrant crop

Abastract

In genetic improvement of mungbean much success has not been achieved due to its recalcitrant nature towards in vitro regeneration. An attempt was made to develop an Agrobacterium-mediated in planta genetic transformation protocol for a locally grown mungbean variety BARI Mung-3 using a screenable marker gene. Two minutes of vacuum infiltration followed by 60 minutes of incubation period in Agrobacterium suspension of Winans

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