Plant Tissue Cult. & Biotech. 29(2): 231-244, 2019 (December)

Agrobacterium rhizogenes-Mediated Transformation of the Critically Endangered Species, Swertia chirayita

Tapojita Samaddar, Sayantika Sarkar and Sumita Jha^*

Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata-700019, India

Key words: Swertia chirayita, Agrobacterium rhizogenes, Hairy root lines, Ri-transformed plants, Swertiamarin

Abastract

Agrobacterium rhizogenes-mediated genetic transformation for the establishment of root culture lines was developed successfully in the critically endangered medicinal plant Swertia chirayita (Roxb.) H. Karst. The key factor for the successfull development of hairy root culture lines was the use of whole micropropagated plants as excised explants of any type did not show any response following infection with A. rhizogenes strains. Maximum root induction frequency (25

Download Full Article