Plant Tissue Cult. & Biotech. 28(2): 147-160, 2018 (December)

Efficient Adventitious Morphogenesis from In vitro Cultures of the Medicinal Plant Cymbopogon schoenanthus

Asmaa Abdelsalam, Kamal Chowdhury¹ and Ahmed El-Bakry^*

Department of Botany, Faculty of Science, Helwan University, Cairo 11795, Egypt

Key words: Biotin, Ca-pantothenate, Maltose, Medicinal plants, Methyl jasmonate

Abastract

Cymbopogon schoenanthus subsp. proximus is a medicinal grass, the source of sesquiterpene proximadiol and is well known in folk medicine as renal anti-spasmodic. The effect of growth regulators, different sugar types, methyl jasmonate and vitamins on de novo organogenesis from seed cultures was studied. Among different concentrations of NAA and BA, 4 mg/l NAA in combination with 0.5 mg/l BA produced 100% callus induction. Both 1.0 and 4.0 mg/l NAA in combination with 0.5 mg/l BA yielded the highest number of shoots. High concentration of sucrose 6% was efficient in root induction from organogenic shoots. Sugar types at a constant concentration of 3% had a significant effect on raising shoot numbers. Different concentrations of methyl jasmonate, biotin and Ca-pantothenate showed a decline in de novo shoot induction, but root formation frequency and numbers were significantly improved with all concentrations used.

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