Plant Tissue Cult. & Biotech. 27(2): 181-187, 2017 (December)

In vitro Morphogenesis of Ornamental Shrubs Camellia japonica and Hydrangea macrophylla

Valentina Malyarovskaya* and Lidiia Samarina

Federal State Budgetary Scientific Institution, Russian Research Institute of Floriculture and Subtropical Crops, 354002 Jan Fabritsius st., 2/28, Sochi, Russia

Key words: In vitro, Morphogensis, Ornamental shrubs, Camellia japonica, Hydrangea macrophylla

Abastract

The effects of genotype, explant source and basal media on shoot growth and callus formation of the mature explants of Camellia japonica and Hydrangea macrophyla were studied. Apical and lateral buds showed higher growth potential compared to meristem in the both species. The best results in three cultivars of C. japonica were obtained on WPM supplemented with 2 mg/l TDZ + 0.5 mg/l Kn + 1 mg/l GA₃. Similarly, best result was observed in two cultivars of H. macrophylla on MS supplemented with 1 mg/l BAP + 0.1 mg/l NAA + 1 mg/l GA₃.

Download Full Article