Plant Tissue Cult. 6(1) : 7-13, 1996

Hypericin from Clonal Propagation of Hypericum canariensis L.

S. mederos, I. Lopez-Bazzocchi¹, A. Ravelo1 and A. Gonzalez¹

Dpto. Biologia Vegetal, Universided de La Laguna, Tenerife, Islas Canarias, Espana.

Key words: Hypericin, In vitro propagation, Medicinal plant

Abastract

Hypericin, a naphtodianthrene with antiretroviral activity, was isolated from the in vitro grown plantlets of Hypericum cannriensis L. and its effects of plant growth regulators on MS and QL.4 culture media on the in vitro proliferation of shoots of this species were examined. The percentage of cultures which produced very good quality of shoots was significantly higher (P = 0.01) on MS medium specially when this culture medium was supplemented with 10^-7 M BAP + 10^-8 M NAA compared to other concentrations of the last mentioned two chemicals. On the other hand, rooting capacity of shoots was improved on a half-strength MS medium supplemented with IBA.

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