Plant Tissue Cult. & Biotech. 26(2): 209-217, 2016 (December)

Genetic Diversity Assessment of Luffa aegyptiaca Landraces Endemic in Egypt Based on Some Molecular Markers

Mohammad Abdel Sttar Al-Tahlawy, Mahmoud Abdel Aziz Ibrahim¹, Mohamed Ahmed Matter*, Mervat El -Sayed Mohamed² and Mahmoud Mohamed Sakr

Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre, 33 El Bohouth St. (former El-Tahrir St.), Dokki, Giza P.O. 12622, Egypt

Key words: Genetic diversity, Luffa aegyptiaca, Endemic landraces, Molecular

Abastract

Luffa aegyptiaca is a popular climbing herb endemic in Egypt. We studied the genetic diversity among ten Luffa landraces (Cairo, Beni Suef, Menoufiya, Damietta, Banha, Aswan, Kafr el-Sheikh, Bir el-Abd, MarsaMatruh and Asyut) collected from different districts in Egypt. The results obtained from DNA fingerprinting revealed that there were polymorphic loci with average percentage of 44.6 among collected landraces whereas polymorphic loci obtained from SDS-PAGE were 23%. Discrimination between landraces was more efficient by using RAPD-PCR marker than total proteins SDS-PAGE which showed a limited level of intraspecific diversity.

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