Plant Tissue Cult. & Biotech. 26(1): 175-185, 2016 (December)

Sink - Source System of In vitro Suspension Culture of Celastrus paniculatus under Regulation of Monochromatic Lights

Vandita Billore¹, Lalit Khatediya and Monica Jain*

Department of Life Sciences, Maharaja Ranjit Singh College of Professional Sciences, Hemkunt campus, Khandwa Road Indore, M.P., India

Key words: Cell suspension culture, Secondary metabolites, Celastrus paniculatus

Abastract

Plants are wonderful resource of bioproducts encompassing significant value to medicines and drug development. The plant cell suspension cultures bear immense potential for production of high-value secondary metabolites and are chosen as alternative sources of raw material for industrial use. In the present study, homogenous cell suspension culture of Celastrus paniculatus a medicinally important plant was established and multifold production of alkaloids and total phenols was obtained under the influence of monochromatic lights. One month old leaf derived friable callus of C. paniculatus was used to raise homogenous suspension culture and kept on rotary shakers in cabinets illuminated with different monochromatic LED lights (Blue, Yellow and Red). The monochromatic lights proved to be a strong abiotic elicitor in driving the production of secondary metabolites so much so that the metabolites were released extracellularly and the medium served as sink or spacious pool for leaked out metabolites from the cell mass. Maximum production and enhancement in alkaloids and phenols (98 and 44.7%, respectively) over control was obtained from cell mass grown under yellow light treatment, followed by blue (64 and 23.7%) and red light (50 and 26%) treatments. Further scale up of secondary metabolite production was hence performed under yellow light conditions, starting from 2.5 gm cell mass suspended in 250 ml of media extended up to 1000 ml culture media for one month. The continuous culture system exhibited remarkable potential of this plant cell system as multifold yield of total alkaloids *Author for correspondence: . 1Department of Biosciences, Pacific Academy of Higher Education and Research University, Pacific Hills, Udaipur, Rajasthan, India. (91.69

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