Plant Tissue Cult. & Biotech. 26(2): 143-149, 2016 (December)

In vitro Conservation of Campanula sclerophylla Kolak - Endemic Endangered Species of Western Caucasus

T. M. Kolomiets, V. I. Malyarovskaya and L. S. Samarina

Laboratory of Plant Biotechnology, Physiology and Biochemistry, Russian Research Institute of Floriculture and Subtropical Crops, Jan Fabritsiusa str., 2/28, 354002 Sochi, Russia

Key words: In vitro conservation, Campanula sclerophylla, Endemic, Endangered species

Abastract

The study was conducted to develop micropropagation and slow-growth conservation protocol for endangered relict plant species Campanula sclerophylla. The best multiplication rate up to 7.2 shoots per explant was obtained on half strength MS supplemented with 3 mg/l BAP and 1 mg/l IAA. The effects of temperature/light conditions, sorbitol and ABA on shoot growth of Campanula sclerophylla were studied. Addition of 3 g/l sorbitol and 5 mg/l ABA inhibited shoot length and multiplication of C. sclerophylla. Explants were stored for 9 months without subculture at 7

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