Plant Tissue Cult. & Biotech. 26(1): 97-104, 2016 (June)

Effect of Glutamine, Biotin and ADP on Micropropa-gation and Growth of Chrysanthemum hybridum, Gerbera jamesonii and Cordyline fruticosa In vitro

L. Samarina*, T. Kolomiets, V. Malyarovskaya, S. Gubaz and N. Platonova

Laboratory of Plant Biotechnology, Physiology and Biochemistry, Russian Research Institute of Floriculture and Subtropical Crops, Jan Fabritsiusa str., 2/28, 354002 Sochi, Russia

Key words: In vitro, Micropropagation, Growth, Microplants

Abastract

The effect of glutamine, biotin and adenosine diphosphate (ADP) on growth and cultivars micropropagation of three ornamental species was investigated. The addition of 10 - 100 mg/l glutamine in culture media significantly increased rate of multiplication in Cordyline fruticosa and 100 mg/l glutamine showed the same effect for Gerbera jamesonii. Addition of glutamine did not show any effect on shoot length, root number and length in all the three species. Addition of 1 - 3 mg/l biotin increased shoot length of Gerbera jamesonii but inhibited shoot length of Cordyline fruticosa and decreased root length of Chrysanthemum hybridum and Gerbera jamesonii. Addition of 5.0 mg/l ADP significantly increased multiplication rate of Cordyline fruticosa and 1.0 mg/l ADP showed similar effect for Gerbera jamesonii.

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