Plant Tissue Cult. & Biotech. 26(1): 25-36, 2016 (June)

Genetic Selection for Salt Tolerance in Some Egyptian Wheat Genotypes (Triticum aestivum L.) via Tissue Culture

Ahmed MS El-Sayed, Hoda MH El-Naggar, Mohamed S Abbas¹ and El-Sayed I Gaber¹

Tissue Culture Laboratory, Department of Botany, National Research Centre, Dokki, Cairo, Egypt

Key words: Triticum aestivum, Callus induction, Plant regeneration

Abastract

Five wheat genotypes and their hybrids under four salinity (sea water) levels were considered for tissue culture and randomly amplified polymorphic DNA (RAPD). The genotypes and the hybrids differed in their ability to callus induction, callus fresh weight and regeneration. Among the genotypes, Sakha 93 (P₁) followed by Line WB19 (P₅) was the most tolerant genotypes for salinity and gave the highest growth rate (46.6%) and (46.3%), respectively while Giza 168 (P₃) was the most sensitive one to salinity with lowest growth rate (26.6%). All hybrids scored higher averages in callus growth rate than their parents. P₁

Download Full Article