Plant Tissue Cult. & Biotech. 26(1): 1-14, 2016 (June)

A Simple Reliable Protocol for Cytogentically Stable Mass Propagation of Ornithogalum virens Lindl.

Saswati Bhattacharya¹, Biswajit Ghosh² and Madhubrata Choudhury*

Department of Biological Sciences, Presidency University, Kolkata 700073, West Bengal, India

Key words: Ornithogalum virens, Micropropagation, Chromosome, RAPD

Abastract

Ornithogalum virens is a plant of horticultural importance but recently researches are focused on its medicinal importance. A simple reliable protocol has been standardized for micropropagation of O. virens to produce a cytologically stable progeny with least polysomatic variation of chromosome number. Among different explants, bulb scales showed the maximum potential for organogenesis. From a single bulb scale 37 bulblets have been produced within 16 weeks of inoculation. A low concentration of auxin with different concentrations of cytokinin is suitable for organogenesis. A higher level of auxin induces callus formation. Bulblet production was the maximum in the medium supplemented with 0.5 mg/l NAA and 2 mg/l 2iP. O. virens normally has a chromosome number of 2n = 6. In nature it shows a common occurrence of polysomaty. The somatic plates that deviate from normal 2n = 6 specially 2n = 12 are of quite common occurrence (approximately 18 - 25% per root tip). Strikingly the tissue culture regenerants show a uniform chromosome number 2n = 6, frequency of polyploidy being negligible. RAPD profile of the explants and its regenerants are almost same. Five primer sets give more number of bands with explants

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