Plant Tissue Cult. & Biotech. 25(2): 165-179, 2015 (December)

Micropropagation and Assessment of Genetic Stability of In Vitro Raised Jojoba (Simmondsia chinensisLink.) Plants Using SCoT and ISSR Markers

S. A. Bekheet, A.M.M. Gabr*, A.A. Reda and M.K. El-Bahr

Department of Plant Biotechnology, National Research Centre, 33 El Bohouth St., Dokki, 12622, Giza, Egypt

Key words: Simmondsia chinensis, Micropropagation, Genetic stability, Sequence repeat analyses

Abastract

This study is aimed at developing an efficient method for micropropagation of true-to-type jojoba plants. Nodal segments were used for in vitro shoots proliferation. Among three concentrations of BA used for multiplication, 1 mg/l BA gave the highest number of shoots. To enhance growth of shoots, combinations of BA and Kn were investigated. The greater value of shoot length and the maximum number of nodes were observed in the medium containing 1 mg/l BA + 1.5 mg/l Kn. Among different medium used to increase the rate of multiplication, the maximum number of shoots was recorded at

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