Plant Tissue Cult. & Biotech. 25(1): 99-102, 2015 (June)

Short Communications

Effect of 5-aminolevulinic Acid on PLB Culture of Cymbidium dayanum In Vitro

Syeda Jabun Nahar¹, Syed Mostafizul Haque and Shimasaki Kazuhiko

Faculty of Agriculture, Kochi University, Nankoku, Kochi 783-8502, Japan

Key words: ALA, Organogenesis, Plant growth regulator, Protocorm-like body

Abastract

5-aminolevulinic acid (5-ALA) has been suggested to be a new natural and environmental friendly regulator, which can be widely used in agriculture. The present study was undertaken to investigate the effect of 5-aminolevulinic acid on organogenesis of Cymbidium dayanum in vitro under white fluorescent tube. PLBs of Cymbidium dayanum were cultured on the modified MS supplemented with 5-ALA at various concentrations (0, 0.1, 1 and 10 mg/l). The addition of 5-ALA in the growth media, showed that 1 mg/l of 5-ALA significantly enhanced the formation of PLB (87%), shoot (73%) and root (47%) to the maximum within 50 days of culture; whereas in the control formation of PLB, shoot and root was the lowest. The result of the present study shown clearly that 5-ALA added to the culture media, acts as a plant growth stimulator to induce PLB, shoot- and root formation of Cymbidium dayanum.

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