Plant Tissue Cult. 5(2) : 137-145, 1995

High frequency in vitro Plantlets Regeneration from an Endangered Tree-Artocarpus lakoocha Roxb.

M. A. Rahman and M. N. Amin

Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Key words: Regeneration, In vitro, Seedling explants, Artocarpus lakoocha

Abastract

The proliferation axillary shoot cultures were established on modified MS medium supplemented with different concentrations of BA and Kn using explants from the in vitro grown seedlings of Artocarpus lakoocha. Explants taken from the seedlings raised in vitro on BA-supplemented medium showed early sprouting and development of axillary shoots than those taken from the seedlings grown o cytokinin-omitted medium. Among the three types of seedling explants, proliferation efficiency of cotyledonary node explants was about three times higher than that of middle node and shoot tip explants. Proliferation medium fortified with 0.5-2.0 mg/1 BA yielded 10-60 axillary shoots within 12 weeks of culture depending on the type of the initial explant. Nodal segments from the in vitro proliferated shoots were subcultured on the proliferation medium for maintaining shoot cultures at callusing thus inhibiting emergence of the roots greatly. Normal rooting, without callusing, was achieved by transferring micro-cuttings to auxin-free medium after one week of auxin treatment or by adding 1.0 mg/1 activated charcoal to the auxin-containing medium.

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