Plant Tissue Cult. & Biotech. 24(2): 279-285, 2014 (December)

Short Communications

Optimization of Lavender (Lavandula angustifolia) Callus Culture

Fatemeh Keykha, Azade Khadem¹*, Abdolreza Bagheri¹, Ahmad Sharifi and Maryam Ameri²

Department Ornamental Plant Biotechnology, Iranian Academic Center for Education, Culture and Research, Branch of Mashhad, Mashhad, Iran

Key words: Lavender (Lavandula angustifolia), Optimization, Callus culture, Light conditions, Explant sources

Abastract

Lavender (Lavandula angustifolia) contains many valuable medicinal components such as linalool and linalyl acetate. This plant species is considered as one of the most important medicinal plants in the world. Enhancement of secondary metabolites production can be achieved through cell culture and genetic. In this study, the results of eight hormonal treatments, carried out with two types of explants under two light conditions were investigated. Maximum callus percentage, both in terms of fresh and dry weight were achieved from leaf explants on MS supplemented with 2 mg/l 2,4-D and 2 mg/l BAP and grown in the dark. Besides genetical properties, the production of plant metabolites, depends on many environmental factors that can affect both quality and quantity of pharmaceuticals. Nowadays, plant tissue culture is known as an efficient alternative method for conventional cultivations that may causes an increase in pharmaceutical metabolites (Stafford 1991, Keykha and Bagheri. 2013). Among various in vitro techniques, callus culture has been successfully established to produce herbal medicines (Kintzios et al. 2003). Dedifferentiation of plant cells can affect the amount of secondary metabolites production in some medicinal species and extraction of these substances from calli has been done in great extent (Patan et al. 2007, Bodhipadma et al. 2012). In addition, callus production is a necessity for cell suspension culture and also this undifferen-tiated cell mass

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