Plant Tissue Cult. & Biotech. 24(2): 273-277, 2014 (December)

Short Communications

Effect of Light Emutting Diode (LED) Lamps and N-acetylglucosamine (NAG) on 0rganogenesis in Protocorm-like Bodies (PLBs) of a Cymbidium Hybrid Cultured In Vitro

Mohammad Mostafa Kamal, Kazuhiko Shimasaki¹ and Nasren Akter²

The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8556 Japan

Key words: LED, N-acetylglucosamine (NAG), Cymbidium, Protocorm-like body

Abastract

Authors investigated the effects of N-acetylglucosamine (NAG) under different LED lights (red, green, and blue) on organogenesis in protocorm-like bodies (PLBs) of a Cymbium hybrid cultured in vitro. Under lighting of green LED, addition of NAG at concentrations of 0.01, 0.1 and 1 mg/l had an potent effect of enhancing PLB numbers. Under red LED treatment, PLB cultures with NAG at concentrations of 0.01 and 0.1 mg/l also showed a higher number and higher rate of PLB formation. Shoot formation in PLB in fortified cultures was promoted under green and red LED light in presence of NAG. Blue LED light had a little effect on the shoot formation in PLB cultures. Fresh weight of cultures was the highest (250 mg) under green LED light in the medium containing 0.01 mg/l NAG. The results suggest that LED light source could be used as an energy efficient light source for control of organogenesis of Cymbidium in vitro and that the presence of NAG in red and green LED plays an important role in the proliferation of PLB and shoot formation. Cymbidium species have been hybridized for over a century to produce plants with flowers of rich texture, color and size that have formed the basis of worldwide flower market. Thus, many attempts have been made to develop better methodologies for Cymbidium micropropagation. These hybrids are gaining in popularity in many countries, especially in Japan. Bartel et al. (2014) proposed a model in which the NAG oligosaccharides modify the architecture of the cell wall. To increase the efficiency of in vitro techniques, culturing conditions such as

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