Plant Tissue Cult. & Biotech. 24(2): 247-262, 2014 (December)

In vitro Regeneration and Somatic Embryogenesis in Citrus

El-Sawy A. Mohamed1, Amina Gomaa² and Nancy Danial

Plant Biotechnology Department, National Research Center, Dokki, Giza, Egypt

Key words: Citrus, In vitro regeneration, Somatic embryogenesis

Abastract

Better results were obtained when stigma explants of variegated lemon and citron were used. After ten months, somatic embryos developed into plantlets at a frequency ranged from 13.3 for lime to 66.7% for lemon. Virus presence was tested by ELISA and RT-PCR. The results indicated that the plantlets regenerated through somatic embryogenesis are CTV-free. RAPD analysis was used to asses the genetic stability of plantlets as compared to the mother plants. The results indicated that most plantlets belong to the respective mother plants and the polymorphism percentage was genotype and explant-dependant.

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