Plant Tissue Cult. 5(2) : 105-111, 1995

Rescue of Hybrid Embryos in Mungbean and Ricebean Cross and their Multiplication through in vitro Technoques

S. K. Bhadra and M. Al-Forkan

Department of Botany, University of Chittagong, Chittagong, Bangladesh

Key words: Rescue, Hybrid embryo, Mungbean, Ricebean

Abastract

By application of 300 mg/1 IAA in lanolin paste around the pedicel and ovary of the pollinated flower and embryo rescue hybrids were obtained unidirectionally from the cross, mungbean (Vigna radiata (L.) Wilczek) and ricebean (Vigna umbrellata (Thunb.) Ohwi and Ohasi) using the former as female parent. The embryos when aseptically cultured on 0.8% (w/v) agar solidified MS medium with 3% (w/v) sucrose at a temperature of 25 ? 2⁰C under cool fluorescent light (1.6 wm^-2) for 14 h in a cycle of 24 h, 50% of them produced seedlings. Different explants of seedlings, namely, shoot apex and epicotyl were cultured on 8% (w/v) agar solidified MS medium supplemented with 3% (w/v) sucrose and different combinations of auxins (2, 4-D, NAA (BA) and cytokinins (Kn). Shoot apical and epicotyl explants with nodes produced multiple shoots only on the medium with 1.0-2.0 mg/1 IBA and 0.5-2.0 mg/1 Kn. When multiple shoots attained a height of 2-3 cm, they were individually transferred to solidified 0.8% (w/v) agar with half strength MS medium containing only 1.5% (w/v) sucrose. When root system developed the complete plants were successfully transferred to pots. Their hybridty was evident from the expression of greenish pigment in the stem, a character of the male parental line.

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