Plant Tissue Cult. 3(1) : 23-27, 1993

Embryogenesis Through Cell Suspension Culture of Forest Tree : Dalbergia latifolia Roxb.

V. Ravishankar Rai

Department of Post-graduate Studies and Research in Botany, University of Mysore, Manasagangotri, Mysore 570 006, India

Key words: Dalbergia latifolia, Indian rosewood, Cell suspension, Embryogenesis, Forest tree

Abastract

Embryogenic cell suspension cultures were established from calli derived from hypocotyl segments of in vitro germinated seedlings of Dalbergia latifolia by placing them in MS liquid medium containing 1 mg/1 NAA and 5 mg/1 BAP. Different stages of development from single celled-to globular proembryo which subsequently transformed into heart or torpedo shaped embryoid were observed. In order to regenerate plants cell suspensions were plated onto MS agar medium containing growth regulators in Petri dishes. Once the shoot buds differentiated, they were transferred to conical flask for further growth and development.

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