Plant Tissue Cult. & Biotech. 22(2): 93-105, 2012 (December)

In vitro Regeneration and Agrobacterium-mediated Transformation of Potato (Solanum tuberosum L.) Cultivars Grown in Mexico

Rose Onamu*, Juan P. Legaria, Jaime C. Sahag?n, Jos? L. Rodr?guez and Joel N. P?rez

Instituto de Horticultura, Departamento de Fitotecnia, Universidad Aut?noma Chapingo, Texcoco, Mexico. Km 38.5, Carretera M?xico-Texcoco. 56230. Chapingo, Estado de M?xico

Key words: Regeneration, Agrobacterium-mediated, Transformation, Potato cultivars

Abastract

Prior to Agrobacterium-mediated genetic transformation in vitro regeneration protocol was established for three potato cultivars (Alfa, Cambray Rosa Morelos and Atlantic) grown in Mexico using leaf, node and internodal explants. Regeneration protocol was developed with or without the intervention of callus. Two potato cultivars, namely, Cambray Rosa Morelos and Alpha were transformed using Agrobacterium tumefaciens strain LBA4404 harboring binary plasmid pBI121 containing the GUS and nptII genes. GUS histochemical assay and PCR analysis were conducted on rooted shoots grown in media without hormones but supplemented with antibiotics. Transformed shoots tested positive through GUS histochemical assay and integration of nptII gene was confirmed by PCR analysis.

Download Full Article