Plant Tissue Cult. & Biotech. 21(1): 1-10, 2011 (June)

Callus Culture of Arceuthobium americanum Nutt. ex Englem. and Explant Response to Varying Auxin and Cytokinin Concentrations

Lyssa L. Martin, Cynthia Ross Friedman* and Ronald G. Smith

Department of Biological Sciences, Thompson Rivers University (TRU), 900 McGill Road, Kamloops, BC, Canada V2C 0C8

Key words: Callus culture, Arceuthobium americanum, Explant, Response, Cytokinin, Insensitivity

Abastract

The lodgepole pine dwarf mistletoe Arceuthobium americanum is a severe pest in coniferous forests of western North America. To facilitate laboratory studies of this obligate parasite, a modified White?s medium was used for in vitro culture; the concentrations of IAA and Kn were varied to determine optimal IAA/Kn ratios. It was found that explant health was related to the concentration of IAA (p = 0.008), but not Kn (p = 0.937), and that explants were healthiest at an IAA/Kn ratio of 0.1. Radicular apices were generated at IAA/Kn ratios of 1.0 or greater, but no shoot organogenesis was observed. These data suggest that A. americanum is more sensitive to auxins than cytokinins. Parasites of plants are known to secrete high levels of cytokinins, which stimulate the host to shuttle nutrients to the infected area, and so we suspect that A. americanum has reduced cytokinin sensitivity.

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