Plant Tissue Cult. 5(1) ; 43-51, 1995

Plant Regeneration of Asparagus asparagoides as Controlled by Explant Type, Growth Regulators and Light Intensity

Biswajit Ghosh and Sumitra Sen

Centre of Advanced Study, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Calcutta 700 019, India

Key words: Asparagus asparagoides, Organogenesis, Light intensity, Growth regulators, Explant type, Plant regeneration

Abastract

For in vitro shoot regeneration, shoot tips, phyllodes, nodes, internodes and roots of Asparagus asparagoides were used as explants. The requirements for induction of callus varied in different explants in relation to the intensity of light, combinations of NAA and Kn, 2, 4-D and 2ip, 2, 4-D and BAP supplements in the basal medium. The amount of BAP or 6-benzylamino-9-[2tetrahydropyranyl] 9H purine (BPA), L-arginine, low level of NAA in the medium and the intensity of light required for the induction of shoots from callus of different explant sources, was found to vary. However, roots could be induced from individual shoots irrespective of explant source in presence of auxin conjugates IAA-L-aspartic acid.

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