Plant Tissue Cult. & Biotech. 20(1): 63-72, 2010 (June)

Rapid in vitro Regeneration and Clonal Propagation of the Fastest Growing Leguminous Tree Albizia falcataria (L.) Fosberg using Leaflet Explant

Nabarun Ghosh*, Don W. Smith, A. B. Das and A. Chatterjee

Department of Life, Earth and Environmental Sciences, West Texas A&M University,

Key words: Albizia falcataria, Leaf explant, Clonal propagation, Cytology

Abastract

Young leaflets were used as the explants for in vitro regeneration of Albizia falcataria (L.) Fosberg without callus intervention. The leaf explants produced in vitro adventitious shoot buds directly on culturing with MS supplemented with BA, IBA (4.0/0.05 mg/l) and 10% coconut milk (v/v). Addition of casein hydrolysate and coconut milk increased the production of shoot buds. The buds produced shoots and roots and showed 66% survival in a field trial. This technique offers an effective way by which large number of genetically stable plants can be produced, maintained, multiplied and transported as disease free propagules or regenerants, safely and economically.

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