Plant Tissue Cult. & Biotech. 18(2): 113-122, 2008 (December)

In vitro Mass Multiplication with Pure Genetic Identity in Anthurium andreanum Lind

S. Gantait, N. Mandal, S. Bhattacharyya¹ and P. K Das¹

Departmen of Biotechnology, Inst rumentation and Environmental Science, B.C.K.V., Mohanpur, W.B.- 741252, India

Key words: Anthurium, In vitro cloning, multiple shoot, genetic indentity

Abastract

A novel protocol for enhanced in vitro multiple shoot induction was developed for Antheurium andreanum Lind. First shoot bud was induced within a week in MS supplemented with 0.1 mg/l NAA and 0.25 mg/l BAP where maximum six buds from single apical bud explant appeared within next 20 day. For multiple shoot proliferation MS with 0.5 mg/1 BAP and 60 mg/1 adenine sulphate (ADS) proved to be best resulting ten multiple shoots per inoculated shoot bud within 50 days. As many as 11 roots per plantlet were producedin in 27 days using MS with 0.5 mg/1 IAA and 2 g/l activated charcoal. Autoclaved sand and intermittent water spraying optimized the primary hardening period of 15 days and then earthen pots filled with sand, soil charcoal and coconut fibre ensured the recovery of 51 well hardened plantlets out of 60 in next 30 days showing 85% success. The Genetic indentity of both ex vitro hardened clones and in vitro sustained clones with their mother plant were tested using 10 ISSR primers, displayed no polymorphism.

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