Plant Tissue Cult. 4(2) : 117-121, 1994

Embryogenesis in Isolated Microspore Cultures of Indian Mustard (Brassica juncea L.)

R. K. Katiyar

National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi-110012, India

Key words: Embryogenesis, Isolated microspore, Indian mustard

Abastract

Direct pollen embryogenesis was achieved in isolated pollen cultueres of two cultivars of Indian mustard (Brassica juncea), in NLN medium containing 13% sucrose. The cultivar Bio-417 produced embryos in the absence of growth hormones but cv. Bio-902 responded only in the presence of BAP and NAA. Whereas cv. Bio-902 showed better response with full strength B₅ wash-medium, cv. Bio-417 responded better when the strength B₅ wash-medium was reduced to half.

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