Plant Tissue Cult. & Biotech. 17(2): 161-172, 2007 (December)

Procedure to Identify and Submit cDNA Sequences to GenBank

George L. Britton Jr., Ahmad S. Islam, Yue Xuan and Kanagasabapathi Sathasivan*

Molecular, Cell and Developmental Biology, School of Biological Sciences, The University of Texas at Austin, USA-78712

Key words: Jute cDNA library, Corchorus olitorius, VecScreen, ORF Finder, Vector Contamination, GenBank, BankIt, Heat shock protein, TAIR WU-BLAST

Abastract

With rapid advances in DNA sequencing and large collection of such information available, it may be helpful to have a simplified procedure for analysis and submission of such sequence information. The process has been described in three steps: (a) data gathering and preparation, (b) data analysis, and (c) data submission to Genbank. The use of NCBI-tools such as VecScreen and ORF finder has been shown to be effective in detecting the vector contaminated sequences and in identifying the segments that code for protein with start- and stop codon, respectively. Another software called A Plasmid Editor is useful in reconstructing the complete DNA sequence of a gene from the sequences of complementary strand. The process of sequence data submission to the GenBank about the gene of interest is described in an easy-to-follow manner, including the final step of receiving an accession number from NCBI.

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