Plant Tissue Cult. & Biotech. 17(2): 131-136, 2007 (December)

In vitro Regeneration of Cabbage (Brassica oleracea L. var. Capitata) through Hypocotyl and Cotyledon Culture

M. K. Munshi, P.K. Roy, M.H. Kabir and G. Ahmed

Institute of Food and Radiation Biology, Atomic Energy Research Establishment, G.P.O. Box-3787, Dhaka-1000, Bangladesh

Key words: In vitro regeneration, Cabbage, Hypocotyl, Cotyledon

Abastract

The best response toward direct regeneration of multiple shoots from seven-day-old seedling was observed on half-strength MS supplemented with 0.5 mg/l BA. Hypocotyl and cotyledon explants produced highest percentage (73 and 66, respectively) of shoots. The maximum number of shoots (12) and the highest shoot length of 5.9 cm were also observed in this medium. On the other hand, indirect regeneration via callus was observed in cotyledonary explants. Maximum percentage of callus formation was observed on MS containing 1.0 mg/l 2,4-D and 0.5 mg/l NAA. Highest frequency of shoot regeneration was achieved on MS fortified with 2.0 mg/l BA and 0.1 mg/l NAA in cotyledon derived callus. Shoot regeneration was not obtained in hypocotyl-derived callus. Shoots rooted well when they were excised individually and implanted in half-strength MS with 0.5 mg/l IBA in which 98% rooting was achieved within 10 - 12 days. The well rooted in vitro raised plantlets were successfully transferred to soil and their survival rate under natural environment was 86%.

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