Plant Tissue Cult. & Biotech. 17(1): 103-108, 2007 (June)

Short Communications

Detection of Maize Intrinsic and Recombinant Cry1Ab Gene Fragment in Genetically Modified Maize

T. Rahman¹, E. H. Chowdhury, A. C. Mondol¹, M. M. Hoque¹ and K. M. Nasiruddin¹

Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh

Key words: Detection, Cry1Ab gene, Maize intrinsic gene, GM maize

Abastract

Polymerase chain reaction (PCR) assay was performed to detect and identify the genetically modified maize (Bt11). Primer pair Bt11 1-5? and Cry1Ab 1-3? detected the region of the insect resistant Cry1Ab gene sequence inserted in GM Bt11 maize and a primer pair ^ZE01-ZE02 were used to detect the maize intrinsic zein (Ze1) gene in maize DNA. The presence of the corresponding DNA segments was specifically detected in GM maize by the designed primers. It was concluded that this method is useful for fast and easy screening of Bt gene in the food products and GM Bt crops.

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