Plant Tissue Cult. & Biotech. 17(1): 65-70, 2007 (June)

Embryogenic Callus Induction and Regeneration of Elite Bangladeshi Indica Rice Cultivars

M. E. Hoque, M. S. Ali and N. H. Karim

Biotechnology Division, Bangladesh Rice Research Institute, Gazipur-1701, Banglad

Key words: Indica rice, Callus induction, Plant regeneration, Genotypic variations

Abastract

Significant variations were observed among six elite Bangladeshi Indica rice cultivars tested in relation to total callus induction frequency (p = 0.017), embryogenic callus formation frequency (p = 0.001) and subsequent plant regeneration responses (p = 0.005). In all the cases, embryogenic callus formation frequency was much more less than the total callus (embryogenic + non-embryonegic) formation frequency. The embryogenic calli derived from mature seed embryos produced green plants, successfully established in soil and produced fertile seeds.

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