Plant Tissue Cult. & Biotech. 16(2): 77-84, 2006 (December)

A Valued Medicinal Plant - Chitrak (Plumbago zeylanica Linn.) : Successful Plant Regeneration Through Various Explants and Field Performance

Binita B. Chaplot, Ashok M. Dave and Yogesh T. Jasrai

Agribiotechnology Laboratory, GSFC Science Foundation, Vigyan Bhavan, Fertilizernagar- 391750, Vadodara, Gujarat, India

Key words: Medicinal plant, Regeneration, Field performance

Abastract

Protocols for plant propagation through axillary bud proliferation and organo-genesis were established for Chitrak - Plumbago zeylanica Linn. (Plumbaginaceae). MS medium with 4.4 mg/l BA and 1.4 mg/l IAA elicited the maximum number of shoots (12 multiple shoots) from nodal explants. Leaf based callus differentiated into more than 30 shoots on MS with 160 mg/l adenine sulphate. The regenerated shoots were rooted on MS with 1.2 mg/l IBA within ten days. Almost, 96% of the rooted shoots survived hardening when transferred to the field. The regenerated plants did not show any morphological change and variation in levels of secondary metabolite when compared with the mother stock.

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