Plant Tissue Cult. 2(2) : 109-113, 1992

Varietal Differences on Plant Regeneration in Grain and Sweet Sorghum

A. M. Rao, K.P.M.S.V. Padmaseree¹, P.B.Kavi Kishor¹ and G.M.Reddy¹

Department of Botany, Kakatiya University, Warangal 506009, India

Key words: Plant regeneration, Grain and Sweet sorghum

Abastract

Kinetin and aspargine suppressed the formation of callus on MS medium from the seeds of both grain and sweet sorghum. Plant regeneration was high in grain sorghum and it ranged from 20 to 87% depending on the genotype. Lower concentrations of Kn and BAP failed to give any organogenic response, but higher concentrations of Kn and BAP were found to be good for regeneration. However. All the eight varieties tested lost their ability to regenerate within 60 to 135 days. Immature seeds gave more organogenic callus when compared to mature seeds. While mature-seed derived callus failed to differentiate, immature-seed derived callus produced plantlets with a frequency of 20-70%.

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