Plant Tissue Cult. 2(2) : 89-95, 1992

Micropropagation of Juvenile and Adult Gmelina arbora

Joysree Sen, M.Shariful Islam, Shyamal K.Roy and S.Hadiuzzaman¹

Department of Botony, Jahanirnagar University, Savar, Dhaka, Bangladesh

Key words: Juvenile, Adult, Gmelina arborea, Micropropagation

Abastract

Successful propagation of juvenile and mature plants of Gmelina arborea Roxb. has been achieved by in vitro methods. Multiple shoot formation was obtained by culturing shoot tips and nodal explants of juvenile source on MS medium containing 1.0 mg/1 BA and 0.1 mg/1 NAA. Explants from mature plants required higher concentrations of BA (1.5 mg/1) and NAA (0.2 mg/1). For inducing axial growth in regenerated shoots, the concentration of BA and NAA were lowered and casein hydrolysate (CH) at 100 mg/1 and coconut milk (CM) at 10% were added to the medium. 100% rooting was achieved on ? MS medium with 1.0 mg/1 each of IBA and NAA in microshoots of juvenile plant source and 2.0 mg/1 each of IBA and NAA in those of mature plant source. About 75% of these plants survived.

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