Plant Tissue Cult. 2(2) : 81-88, 1992

Investigation into the Possible Causes for Failure of In vitro Regeneration in Mungbean

Mondira Bose, R.H.Sarker, M.Imdadul Hoque and M.M.Haque

Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Key words: Mungbean, Vigna radiata, Regeneration, Fluorescent microscopy, Cell suspension culture

Abastract

Plant regeneration was tried from different explants of Vigna radiata, namely, shoot tip, epicotyl, hypocotyls, cotyledonary node and leaf segments. Leaf explants callused best when cultured on MS medium containing 1.0 mg/1 2,4-D+0.5 mg/1 Kn. Only proliferation of callus took place when they were cultured on a medium specially prepared to induce regeneration. Microscopic observations revealed the presence of organogenic cell lines only at their early stages; however, at later stages these cells turned into thick-walled cells, which did not differentiate. Fluorescent microscopic study of fresh suspension culture callus cells showed presence of newly synthesized starch bodies and cells of different shapes and sizes. From six weeks onward the cells lost starch grains, became partially vacuolated finally giving rise to empty cells.

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