Plant Tissue Cult : 2(1) : 41-47,1992

SOME FACTORS ENHANCING MICROPROPAGATION OF CHRYSANTHEMUM MORIFOLIUM Ram.

Y.P.S.Bajaj¹, M.M.S.Sidhu² and A.P.S.Gill

Punjab Agricultural University, Ludhiana-141004, India

Key words:

Abastract

Plants and multiple shoots have been regenerated from in vitro grown shoot tips, segments of stem, capitulum, peduncle, and callus culture of a slow-multiplying summer flowering cv. Fredyule of Chrysanthemum morifolium. Of different explants, the best response was obtained from excised segments of inflorescences grown on Murashige and Skoog?s medium supplemented indole-3-acetic acid and kinetin. The in vitro-derived plants acclimated better when they were transferred to pots containing soil mixture of sand, leaf mould and farmyard manure, covered with polythene bags and left to grow for a week. The terminal cuttings from these plants were utilized for further multiplication in pots. The plants thus obtained produced normal flowers. A combination of tissue culture and the conventional cuttings method has been successfully practiced for the propagation of Chrysanthemum.

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