Plant Tissue Cult. 2(1): 27-30, 1992

IN VITRO ENHANCED PROLIFERATION OF SHOOTS AND REGENERATION OF PLANTS FROM EXPLANTS OF JACKFRUIT TREES.

M.N. Amin

Department of Botany, Rajshahi University, Rajshahi-6205, Bangladesh

Key words:

Abastract

Apical buds containing primordial inflorescences collected from the footstalks of adult trees produced tiny immature inflorescences (7-15 mm long) within 6 weeks of culture on MS medium supplemented with 1 mg/1 BA. Upon isolation, sectioning and subculturing on similar medium containing 0.5-1 mg/1 BA alone or with 0.1 mg/1 NAA, each section of tiny inflorescences produced uniformly growing shoot buds along their full length within 4 weeks of incubation. The buds developed into rootable shoots (5-15/culture) 4 weeks after additional subculture. Shoots could be multiplied (3-8-fold at every 4 weeks) through the enhanced growth of axillary buds on nodal segments removed from the newly developed in vitro shoots on medium having 1 mg/1 BA only. The microcuttings were rooted with 90% success using 2 mg/1 BA in half strength MS medium and by incubating them at 30 C under complete darkness for initial 7-10 days. Employing the present technique an estimated 500 plants could be regenerated from a single explant within 24 weeks.

Download Full Article