Plant Tissue Cult. 2(1): 21-25, 1992

IN VITRO CULTURE OF CARICA PAPAYA L. IV. EFFECT OF GROWTH REGULATORS ON INDUCTION, GROWTH AND LONG TERM MAINTENANCE OF CALLUS CULTURE.

S. M. Rahman, M. Hossain and O.I. Joarder and R. Islam

Department of Botany, Rajshahi University, Rajshahi-6205, Bangladesh

Key words:

Abastract

In vitro callus culture was induced by culturing petiole segments of papaya (Carica papaya L.) on MS medium supplemented with various combinations and concentrations of NAA, 2,4-D, BA + NAA and BA + 2,4-D with or without coconut water (CW). Coconut water markedly increased callus growth when used with any of the two auxins. Optimum callus growth was observed on the medium supplemented with 0.5 mg/1 2,4-D + 15% CW. The same medium formulation was found to be the best for long term maintenance of callus tissue. Callus culture was maintained over 20 subcultures without showing any loss of vigour.

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