Plant Tissue Cult. 2(1): 15-20, 1992

CELL SUSPENSION CULTURES IN THREE VARIETIES OF JUTE (CORCHORUS SPP.)

Sunil K. Halder and Zeba I. Seraj

Department of Biochenistry, Dhaka University, Dhaka-1000, Bangladesh

Key words:

Abastract

Friable calli of Corchorus capsularis var. D-154, var. CVL-1 and C. olitorius var. 0-4 were obtained by subculturing compact calli. On media MS+BAP+tyr and MS+BAP+tyr+NDGA hypocotyl segments were used as inoculum for suspension cultures. The friability of callus was greater in media containing NDGA. Optimum growth curves were obtained for suspension cultures in MS+BAP+tyr+2, 4-D. Maximum growth of cells in the latter medium was obtained in 8-11 days after which the suspension was subcultured. Organogenic nature of the cells in suspension was checked using specific activity of the enzyme glyoxalase-I as a marker. 2,4-D, when added as a growth factor to the medium decreased the organogenic potentiality of cells in suspension. A minimum amount of 0.25 mg/1 of 2,4-D was required to maintain the suspension cultures. Some of the cells showed the presence of starch granules suggesting organogenic nature of cells. Plating of such cells on 2,4-D free semisolid MS resulted only in root differentiation.

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