Plant Tissue Cult. 2(1): 7-13, 1992

AXILLARY SHOOT PROLIFERATION AND ADVENTITIOUS ROOTING IN VITRO OF CARAMBOLA (AVERRHOA CARAMBOLA L.)

M.N.Amin, M.A.Razzaque¹and Shamsia Akhter

Department of Botany, Rajshahi University, Rajshahi-6205, Bangladesh

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Abastract

Proliferating cultures of axillary shoots were established on MS and modified MS media from shoot explants in vitro raised seedlings of A.carambola with different concentrations of BA and kinetin. Seedlings grown on medium cultures of axillary shoots were established on MS and containing BA 0.5mg/1 developed into bushy shoots with reduced hypocotyl and roots. Nodal segments from these seedlings were found to be the best explants for axillary shoot formation on agar gelled MS medium with ? major salts and 0.5-1.0mg/1 of BA. On this medium axillary buds showed sprouting within one week of incubation. By sequential reculturing and subculturing 20-30 usable shoots (>1cm length) could be produced from 2-node shoot segments after 10-12 weeks of culture. Microcuttings taken from in vitro proliferated shoots were rooted on ? strength MS medium having 0.1-0.5mg/1 of IBA and/or NAA. Maximum rooting on (90%) with 2-4 roots per cutting was achieved at 0.2mg/1 of IBA on the same medium.

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