Plant Tissue Cult. & Biotech. 15(2): 135-144, 2005 (December)

In vitro Direct Regeneration of Three Indigenous Chickpea (Cicer arietinum L.) Varieties of Bangladesh

R. H. Sarker, Tarannum Ferdous and M. I. Hoque

Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Key words: Indigenous chickpea, Direct organogenesis

Abastract

An in vitro direct plant regeneration protocol was developed for three locally grown chickpea (Cicer arietinum L.) varieties of Barichhola, namely BCh-2, BCh-4 and BCh-5. The best response towards multiple shoot regeneration was achieved from cotyledonary nodal explants on MSB5 containing 4? MS micro-salts, 3.0 mg/l BAP and 0.04 mg/l NAA in all the three varieties of chickpea. In decapitated mature embryo axes explant best shoot regeneration was obtained on MS supplemented with 0.5 mg/l BAP, 0.5 mg/l Kn, 0.2 mg/l NAA, 4 mg/l tyrosine, 880 mg/l CaCl2 and 3800 mg/l KNO3 in BCh-4 and BCh-5. Shoots regenerated on 1.0 mg/l Kn supplemented medium rooted efficiently on MS containing 0.2 mg/l BAP and 0.5 mg/ NAA in all the three varieties. Micrografting technique has been found to be a good alternative to in vitro rooting.

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