PlantTissue Cult. 4(1) : 59-67, 1994

Vegetative Propagation of Eucalyptus tereticornis Sm. Through Tissue Culture

R.I.S. Gill, S.S. Gill and S.S. Gosal¹

Department of Forestry & Natural Resources, Punjab Agricultural University, Ludhiana-141 004, India

Key words: Eucalyptus tereticornis Sm., Germplasm, Heterozygous, Multiplication, Multiple shoots, Asexual multiplication

Abastract

Nodal segments proved to be the best explant for asexual multiplication of Eucalyptus tereticornis Sm. MS basal medium supplemented with lower concentration (0.5 mg/1) of both BAP and kinetin was found better in terms of multiple shoot formation as compared to higher concentration (1.0 mg/1) of these growth regulators. Similar response was observed in both solidified agar and liquid media. The frequent shoot bud multiplication inhibited elongation of shoots. Subculturing of shoot bud clumps on the elongation medium (MS + BAP (0.5 mg/1) + IBA (1.0 mg/1) improved multiplication rate. The maximum rooting (73.5%) was observed on ? MS medium supplemented with NAA (0.2 mg/1) and IBA (1 mg/1). The maximum survival per cent (58.1) in the open environment was obtained when plantlets were transferred on to Soilrite mix. Vermiculite (exfoliated) proved to be the second best with 40.8% survival.

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